PCR versus hybridization for detecting virulence genes of enterohemorrhagic Escherichia coli.

ABSTRACT: We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. Both methods showed 100% of the chromosomal and phage genes: eae, stx, and stx2. PCR did not detect 4%-20% of hybridizable plasmid genes: hlyA, katP, espP, toxB, open reading frame (ORF) 1, and ORF2.

SUBMITTER: Gerrish RS 

PROVIDER: S-EPMC2828064 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature