Genetic response to metabolic fluctuations: correlation between central carbon metabolism and DNA replication in Escherichia coli.

ABSTRACT:

Background

Until now, the direct link between central carbon metabolism and DNA replication has been demonstrated only in Bacillus. subtilis. Therefore, we asked if this is a specific phenomenon, characteristic for this bacterium and perhaps for its close relatives, or a more general biological rule.

Results

We found that temperature-sensitivity of mutants in particular genes coding for replication proteins could be suppressed by deletions of certain genes coding for enzymes of the central carbon metabolism. Namely, the effects of dnaA46(ts) mutation could be suppressed by dysfunction of pta or ackA, effects of dnaB(ts) by dysfunction of pgi or pta, effects of dnaE486(ts) by dysfunction of tktB, effects of dnaG(ts) by dysfunction of gpmA, pta or ackA, and effects of dnaN159(

SUBMITTER: Maciag M 

PROVIDER: S-EPMC3080795 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature