Comparison of the specificities and efficacies of primers for aromatic dioxygenase gene analysis of environmental samples.
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ABSTRACT:
SUBMITTER: Iwai S
PROVIDER: S-EPMC3127589 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
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Comparison of the specificities and efficacies of primers for aromatic dioxygenase gene analysis of environmental samples.
Iwai Shoko S Johnson Timothy A TA Chai Benli B Hashsham Syed A SA Tiedje James M JM
Applied and environmental microbiology 20110415 11
Aromatic dioxygenase genes have long been of interest for bioremediation and aromatic carbon cycling studies. To date, 115 primers and probes have been designed and used to analyze dioxygenase gene diversities in environmental samples. Here we analyze those primers' specificities, coverages, and PCR product lengths compared to known aromatic dioxygenase genes based on in silico analysis as well as summarize their differing advantages or effectiveness from over 50 reported experimental studies. W ...[more]
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