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Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

ABSTRACT: High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 ?M and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

SUBMITTER: Unkles SE 

PROVIDER: S-EPMC3232726 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature

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Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Unkles Shiela E SE   Symington Vicki F VF   Kotur Zorica Z   Wang Ye Y   Siddiqi M Yaeesh MY   Kinghorn James R JR   Glass Anthony D M AD  

Eukaryotic cell 20111021 12

High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the  ...[more]

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