Improved culture-based isolation of differentiating endothelial progenitor cells from mouse bone marrow mononuclear cells.

ABSTRACT: Numerous endothelial progenitor cell (EPC)-related investigations have been performed in mouse experiments. However, defined characteristics of mouse cultured EPC have not been examined. We focused on fast versus slow adherent cell population in bone marrow mononuclear cells (BMMNCs) in culture and examined their characteristics. After 24 h-culture of BMMNCs, attached (AT) cells and floating (FL) cells were further cultured in endothelial differentiation medium separately. Immunological and molecular analyses exhibited more endothelial-like and less monocyte/macrophage-like characteristics in FL cells compared with AT cells. FL cells formed thick/stable tube and hypoxia or shear stress overload further enhanced these endothelial-like features with increased angiogenic cytokine/growth facto

SUBMITTER: Sekiguchi H 

PROVIDER: S-EPMC3247221 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature