Profiling of differentially expressed genes using suppression subtractive hybridization in an equine model of chronic asthma.

ABSTRACT:

Background

Gene expression analyses are used to investigate signaling pathways involved in diseases. In asthma, they have been primarily derived from the analysis of bronchial biopsies harvested from mild to moderate asthmatic subjects and controls. Due to ethical considerations, there is currently limited information on the transcriptome profile of the peripheral lung tissues in asthma.

Objective

To identify genes contributing to chronic inflammation and remodeling in the peripheral lung tissue of horses with heaves, a naturally occurring asthma-like condition.

Methods

Eleven adult horses (6 heaves-affected and 5 controls) were studied while horses with heaves were in clinical remission (Pasture), and during disease exacerbation induced by a 30-day natural antigen ch

SUBMITTER: Lavoie JP 

PROVIDER: S-EPMC3250435 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature