Comprehensive analysis of protein modifications by top-down mass spectrometry.

ABSTRACT: Mass spectrometry (MS)-based proteomics is playing an increasingly important role in cardiovascular research. Proteomics includes identification and quantification of proteins and the characterization of protein modifications, such as posttranslational modifications and sequence variants. The conventional bottom-up approach, involving proteolytic digestion of proteins into small peptides before MS analysis, is routinely used for protein identification and quantification with high throughput and automation. Nevertheless, it has limitations in the analysis of protein modifications, mainly because of the partial sequence coverage and loss of connections among modifications on disparate portions of a protein. An alternative approach, top-down MS, has emerged as a powerful tool for the analysis

SUBMITTER: Zhang H 

PROVIDER: S-EPMC3320739 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature