Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.

ABSTRACT: One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.

SUBMITTER: Donnelly DP 

PROVIDER: S-EPMC6719561 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature