γ-Aminobutyric acid (GABA) signalling in human pancreatic islets is altered in type 2 diabetes.

ABSTRACT:

Aims/hypothesis

γ-Aminobutyric acid (GABA) is a signalling molecule in the interstitial space in pancreatic islets. We examined the expression and function of the GABA signalling system components in human pancreatic islets from normoglycaemic and type 2 diabetic individuals.

Methods

Expression of GABA signalling system components was studied by microarray, quantitative PCR analysis, immunohistochemistry and patch-clamp experiments on cells in intact islets. Hormone release was measured from intact islets.

Results

The GABA signalling system was compromised in islets from type 2 diabetic individuals, where the expression of the genes encoding the α1, α2, β2 and β3 GABA(A) channel subunits was downregulated. GABA originating within the islets evoked tonic currents in th

SUBMITTER: Taneera J 

PROVIDER: S-EPMC3369140 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature