Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

ABSTRACT: Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid chromatography mass spectrometry to compare the nuclear, cytoplasmic and membrane associated proteomes of multipotent (MSC) (CD105+) and non-multipotent (CD105-) stromal cells. Among the 25 most reliably identified proteins, 10 were verified by both real-time PCR and Western Blot to be highly enriched, in CD105+ cells and were members of distinct biological pathways and functional networks. Five of these proteins were also identified as potentially expressed in human MSC derived from both standar

SUBMITTER: Rosu-Myles M 

PROVIDER: S-EPMC3374805 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature