Role of histamine and its receptor subtypes in stimulation of conjunctival goblet cell secretion.

ABSTRACT:

Purpose

The purpose of this study was to determine the effect of histamine and its receptors on goblet cell secretion.

Methods

Cultured rat and human goblet cells were grown in RPMI 1640. Goblet cell secretion of high molecular weight glycoconjugate was measured by an enzyme-linked lectin assay. Cultured rat goblet cells were homogenized and either RNA was isolated for RT-PCR or proteins were isolated for Western blot analysis for presence of histamine receptors subtypes H₁ through H₄. The localization of these receptors was determined in rat and human goblet cells by immunofluorescence microscopy.

Results

Histamine stimulated goblet cell secretion in a concentration- and time-dependent manner. All four histamine receptors were present in cultured rat and human goblet

SUBMITTER: Hayashi D 

PROVIDER: S-EPMC3376076 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature