Dataset Information
Production and crystallization of ?-phosphoglucomutase from Lactococcus lactis.
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ABSTRACT:
SUBMITTER: Nogly P
PROVIDER: S-EPMC3433211 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Publications
Production and crystallization of α-phosphoglucomutase from Lactococcus lactis.
Acta crystallographica. Section F, Structural biology and crystallization communications 20120831 Pt 9
α-Phosphoglucomutase (α-PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6-phosphate and α-glucose 1-phosphate. The gene encoding α-PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour-diffusion and seeding techniques. Optimized ...[more]