A Rex family transcriptional repressor influences H2O2 accumulation by Enterococcus faecalis.

ABSTRACT: Rex factors are bacterial transcription factors thought to respond to the cellular NAD(+)/NADH ratio in order to modulate gene expression by differentially binding DNA. To date, Rex factors have been implicated in regulating genes of central metabolism, oxidative stress response, and biofilm formation. The genome of Enterococcus faecalis, a low-GC Gram-positive opportunistic pathogen, encodes EF2638, a putative Rex factor. To study the role of E. faecalis Rex, we purified EF2638 and evaluated its DNA binding activity in vitro. EF2638 was able to bind putative promoter segments of several E. faecalis genes in an NADH-responsive manner, indicating that it represents an authentic Rex factor. Transcriptome analysis of a ΔEF2638 mutant revealed that genes likely to be involved in anaerobic meta

SUBMITTER: Vesic D 

PROVIDER: S-EPMC3624565 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature