Ontology highlight
ABSTRACT:
SUBMITTER: Chung JY
PROVIDER: S-EPMC3777740 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Chung Joon-Yong JY Lee Seo-Jin SJ Kris Ylaya Y Braunschweig Till T Traicoff June L JL Hewitt Stephen M SM
Proteomics. Clinical applications 20080826 10-11
Proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue offers significant diagnostic utility but is complicated due to the high level of covalently crosslinked proteins arising from formalin fixation. To address these challenges, we developed a reliable protein extraction method for FFPE tissue, based on heat-induced antigen retrieval within a pressure cooker. The protein extraction yield from archival FFPE tissue section is approximately 90% of that recovered from frozen tissue. T ...[more]