Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production.

ABSTRACT:

Background

Polyketides are one of the most important classes of secondary metabolites and usually make good drugs. Currently, heterologous production of fungal polyketides for developing a high potential industrial application system with high production capacity and pharmaceutical feasibility was still at its infancy. Pichia pastoris is a highly successful system for the high production of a variety of heterologous proteins. In this work, we aim to develop a P. pastoris based in vivo fungal polyketide production system for first time and evaluate its feasibility for future industrial application.

Results

A recombinant P. pastoris GS115-NpgA-ATX with Aspergillus nidulans phosphopantetheinyl transferase (PPtase) gene npgA and Aspergillus terrus 6-methylsalicylic acid (6-MSA)

SUBMITTER: Gao L 

PROVIDER: S-EPMC3847973 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature