MRNA expression, functional profiling and multivariate classification of colon biopsy specimen by cDNA overall glass microarray.

ABSTRACT:

Aim

To understand the local pathophysiological alterations and gene ontology-based functional classification of colonic biopsies into inflammatory and neoplastic diseases.

Methods

Total RNA was extracted from frozen biopsies and amplified by T7-method. Expression profile was evaluated by Atlas Glass 1K microarrays. After microarray quality control, applicable data were available from 10 adenomas, 6 colorectal adenocarcinomas (CRCs), and 6 inflammatory bowel diseases (IBDs). Multivariate statistical and cell functional analyses were performed. Real-time RT-PCR and immunohistochemistry were used for validation.

Results

Discriminant analysis of selected genes, could correctly reclassify all 22 samples using 4 parameters (heat shock transcription factor-1, bystin-like, ca

SUBMITTER: Galamb O 

PROVIDER: S-EPMC4087344 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature