Gene expression analysis of primary normal human hepatocytes infected with human hepatitis B virus.

ABSTRACT:

Aim

To find the relationship between hepatitis B virus (HBV) and hepatocytes during the initial state of infection by cDNA microarray.

Methods

Primary normal human hepatocytes (PNHHs) were isolated and infected with HBV. From the PNHHs, RNA was isolated and inverted into complement DNA (cDNA) with Cy3- or Cy5- labeled dUTP for microarray analysis. The labeled cDNA was hybridized with microarray chip, including 4224 cDNAs. From the image of the microarray, expression profiles were produced and some of them were confirmed by RT-PCR, immunoblot analysis, and NF-kappaB luciferase reporter assay.

Results

From the cDNA microarray, we obtained 98 differentially regulated genes. Of the 98 genes, 53 were up regulated and 45 down regulated. Interestingly, in the up regulated ge

SUBMITTER: Ryu HM 

PROVIDER: S-EPMC4087401 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature