Identification of novel gene signatures in patients with atopic dermatitis complicated by eczema herpeticum.

ABSTRACT:

Background

A subset of patients with atopic dermatitis (AD) is prone to disseminated herpes simplex virus (HSV) infection (ie, atopic dermatitis with a history of eczema herpeticum [ADEH+]). Biomarkers that identify ADEH+ are lacking.

Objective

We sought to search for novel ADEH+ gene signatures in PBMCs.

Methods

An RNA-sequencing approach was applied to evaluate global transcriptional changes by using PBMCs from patients with ADEH+ and patients with atopic dermatitis without a history of eczema herpeticum (ADEH-). Candidate genes were confirmed by means of quantitative PCR or ELISA.

Results

PBMCs from patients with ADEH+ had distinct changes to the transcriptome when compared with those from patients with ADEH- after HSV-1 stimulation: 792 genes were different

SUBMITTER: Bin L 

PROVIDER: S-EPMC4186924 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature