Multiple species of Trichosporon produce biofilms highly resistant to triazoles and amphotericin B.

ABSTRACT: Invasive infections caused by Trichosporon spp. have increased considerably in recent years, especially in neutropenic and critically ill patients using catheters and antibiotics. The genus presents limited sensitivity to different antifungal agents, but triazoles are the first choice for treatment. Here, we investigated the biofilm production and antifungal susceptibility to triazoles and amphotericin B of 54 Trichosporon spp. isolates obtained from blood samples (19), urine (20) and superficial mycosis (15). All isolates and 7 reference strains were identified by sequence analysis and phylogenetic inferences of the IGS1 region of the rDNA. Biofilms were grown on 96-well plates and quantitation was performed using crystal violet staining, complemented with Scanning Electron Microscopy (SE

SUBMITTER: Iturrieta-Gonzalez IA 

PROVIDER: S-EPMC4215839 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature