Granulocyte colony-stimulating factor prevents loss of spermatogenesis after sterilizing busulfan chemotherapy.

ABSTRACT:

Objective

To determine whether granulocyte colony-stimulating factor (G-CSF) could prevent loss of spermatogenesis induced by busulfan chemotherapy via protection of undifferentiated spermatogonia, which might serve as an adjuvant approach to preserving male fertility among cancer patients.

Design

Laboratory animal study.

Setting

University.

Animal(s)

Laboratory mice.

Intervention(s)

Five-week-old mice were treated with a sterilizing busulfan dose and with 7 days of G-CSF or vehicle treatment and evaluated 10 weeks later (experiment 1) or 24 hours after treatment (experiment 2).

Main outcome measure(s)

Experiment 1: testis weights, epididymal sperm counts, testis histology. Experiment 2: PLZF immunofluorescent costaining with apoptotic markers. Mo

SUBMITTER: Benavides-Garcia R 

PROVIDER: S-EPMC4282609 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature