Identification of serum sirtuins as novel noninvasive protein markers for frailty.

ABSTRACT: Frailty has emerged as a major health issue among older patients. A consensus on definition and diagnosis is yet to be achieved. Various biochemical abnormalities have been reported in frailty. Activation of sirtuins, a conserved family of NAD-dependent proteins, is one of the many mimics of calorie restriction which improves lifespan and health in experimental animals. In this cross-sectional study, we assessed the circulating sirtuin levels in 119 (59.5%) nonfrail and 81 (40.5%) frail individuals, diagnosed by Fried's criteria. Serum SIRT1, SIRT2, and SIRT3 were estimated by surface plasmon resonance (SPR) and Western blot. Serum sirtuins level in mean+SD; SIRT1 (nonfrail -4.67 ± 0.48 ng/μL; frail - 3.72 ± 0.48 ng/μL; P < 0.0001), SIRT2 (nonfrail - 15.18 ± 2.94 ng/μL; frail - 14.19 ± 2.6

SUBMITTER: Kumar R 

PROVIDER: S-EPMC4326933 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature