Unknown

Dataset Information

0

Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq.

ABSTRACT: Eukaryotic genomes encode a large number of RNA-binding proteins, which play critical roles in many aspects of gene regulation. To functionally characterize these proteins, a key step is to map their interactions with target RNAs. UV crosslinking and immunoprecipitation coupled with high-throughput sequencing has become the standard method for this purpose. Here we describe the detailed procedure that we have used to characterize the protein-RNA interactions of the mRNA 3' processing factors.

SUBMITTER: Yao C 

PROVIDER: S-EPMC4412157 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq.

Yao Chengguo C   Weng Lingjie L   Shi Yongsheng Y  

Methods in molecular biology (Clifton, N.J.) 20140101

Eukaryotic genomes encode a large number of RNA-binding proteins, which play critical roles in many aspects of gene regulation. To functionally characterize these proteins, a key step is to map their interactions with target RNAs. UV crosslinking and immunoprecipitation coupled with high-throughput sequencing has become the standard method for this purpose. Here we describe the detailed procedure that we have used to characterize the protein-RNA interactions of the mRNA 3' processing factors. ...[more]

Similar Datasets

Unknown Epitranscriptomic mapping of RNA modifications at single-nucleotide resolution using rhodamine sequencing (Rho-seq).
| S-EPMC9092995 | biostudies-literature
Unknown Analysis of CLIP and iCLIP methods for nucleotide-resolution studies of protein-RNA interactions.
| S-EPMC4053741 | biostudies-literature
Unknown Improved binding site assignment by high-resolution mapping of RNA-protein interactions using iCLIP.
| S-EPMC4918375 | biostudies-literature
Unknown PureCLIP: capturing target-specific protein-RNA interaction footprints from single-nucleotide CLIP-seq data.
| S-EPMC5746957 | biostudies-literature
Unknown Mapping in vivo protein-RNA interactions at single-nucleotide resolution from HITS-CLIP data.
| S-EPMC3400429 | biostudies-literature
Unknown High-resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-Seq).
| S-EPMC6663860 | biostudies-literature
Unknown Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution.
| S-EPMC3443142 | biostudies-literature
Unknown Function annotation of the rice transcriptome at single-nucleotide resolution by RNA-seq.
| S-EPMC2928502 | biostudies-literature
Unknown RNA-Seq reveals differential gene expression in Staphylococcus aureus with single-nucleotide resolution.
| S-EPMC3792026 | biostudies-literature
Unknown A Transcriptome Map of Actinobacillus pleuropneumoniae at Single-Nucleotide Resolution Using Deep RNA-Seq.
| S-EPMC4809551 | biostudies-literature