The BisPCR(2) method for targeted bisulfite sequencing.

ABSTRACT:

Background

DNA methylation has emerged as an important regulator of development and disease, necessitating the design of more efficient and cost-effective methods for detecting and quantifying this epigenetic modification. Next-generation sequencing (NGS) techniques offer single base resolution of CpG methylation levels with high statistical significance, but are also high cost if performed genome-wide. Here, we describe a simplified targeted bisulfite sequencing approach in which DNA sequencing libraries are prepared following sodium bisulfite conversion and two rounds of PCR for target enrichment and sample barcoding, termed BisPCR(2).

Results

We have applied the BisPCR(2) technique to validate differential methylation at several type 2 diabetes risk loci identified in gen

SUBMITTER: Bernstein DL 

PROVIDER: S-EPMC4522100 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature