Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse.

ABSTRACT:

Objective

Our objectives were to determine the expression of EVC2 in craniofacial tissues and investigate the effect of Evc2 deficiency on craniofacial bones using Evc2 knockout (KO) mouse model.

Design

Evc2 KO mice were generated by introducing a premature stop codon followed by the Internal Ribosomal Entry Site fused to β-galactosidase (LacZ). Samples from wild-type (WT), heterozygous (Het) and homozygous Evc2 KO mice were prepared. LacZ staining and immunohistochemistry (IHC) with anti-β-galactosidase, anti-EVC2 and anti-SOX9 antibodies were performed. The craniofacial bones were stained with alcian blue and alizarin red.

Results

The LacZ activity in KO was mainly observed in the anterior parts of viscerocranium. The Evc2-expressing cells were identified in many ca

SUBMITTER: Badri MK 

PROVIDER: S-EPMC4903910 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature