Peroxisome Proliferator-Activated Receptor ? Activity is Required for Appropriate Cardiomyocyte Differentiation.

ABSTRACT: OBJECTIVE:Peroxisome proliferator-activated receptor ? (PPAR?) is a member of the PPAR nuclear receptor superfamily. Although PPAR? acts as a master transcription factor in adipocyte differentiation, it is also associated with a variety of cell functions including carbohydrate and lipid metabolism, glucose homeostasis, cell proliferation and cell differentiation. This study aimed to assess the expression level of PPAR? in order to address its role in cardiac cell differentiation of mouse embryonic stem cells (mESCs). MATERIALS AND METHODS:In this an intervening study, mESCs were subjected to cardiac differentiation. Total RNA was extracted from the cells and quantitative real time polymerase chain reaction (qPCR) was carried out to estimate level of gene expression. Furthermore, the requirement of PPAR? in cardiac differentiation of mESCs, during cardiac progenitor cells (CPCs) formation, was examined by applying the respective agonist and antagonist. RESULTS:The obtained data revealed an elevation in the expression level of PPAR? during spontaneous formation of CPCs and cardiomyocytes. Our results indicated that during CPC formation, PPAR? inactivation via treatment with GW9662 (GW) reduced expression of CPC and cardiac markers. CONCLUSION:We conclude that PPAR? modulation has an effective role on cardiac differentiation of mESCs at the early stage of cardiomyogenesis.

SUBMITTER: Peymani M 

PROVIDER: S-EPMC4988421 | biostudies-literature | 2016 Jul-Sep

REPOSITORIES: biostudies-literature