Acute Catecholamine Exposure Causes Reversible Myocyte Injury Without Cardiac Regeneration.

ABSTRACT:

Rationale

Catecholamines increase cardiac contractility, but exposure to high concentrations or prolonged exposures can cause cardiac injury. A recent study demonstrated that a single subcutaneous injection of isoproterenol (ISO; 200 mg/kg) in mice causes acute myocyte death (8%-10%) with complete cardiac repair within a month. Cardiac regeneration was via endogenous cKit(+) cardiac stem cell-mediated new myocyte formation.

Objective

Our goal was to validate this simple injury/regeneration system and use it to study the biology of newly forming adult cardiac myocytes.

Methods and results

C57BL/6 mice (n=173) were treated with single injections of vehicle, 200 or 300 mg/kg ISO, or 2 daily doses of 200 mg/kg ISO for 6 days. Echocardiography revealed transiently increase

SUBMITTER: Wallner M 

PROVIDER: S-EPMC5026617 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature