Obesity but not high-fat diet impairs lymphatic function.

ABSTRACT:

Background/objectives

High-fat diet (HFD)-induced obesity has significant negative effects on lymphatic function, but it remains unclear whether this is a direct effect of HFD or secondary to adipose tissue deposition.

Methods

We compared the effects of HFD on obesity-prone and obesity-resistant mice and analyzed lymphatic function in vivo and in vitro.

Results

Only obesity-prone mice had impaired lymphatic function, increased perilymphatic inflammation and accumulation of lipid droplets surrounding their lymphatic endothelial cells (LECs). LECs isolated from obesity-prone mice, in contrast to obesity-resistant animals, had decreased expression of VEGFR-3 and Prox1. Exposure of LECs to a long-chain free fatty acid increased cellular apoptosis and decreased VEGFR-3 exp

SUBMITTER: Garcia Nores GD 

PROVIDER: S-EPMC5050064 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature