A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research.

ABSTRACT: Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100

SUBMITTER: Kockel L 

PROVIDER: S-EPMC5068927 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature