Efficient recellularisation of decellularised whole-liver grafts using biliary tree and foetal hepatocytes.

ABSTRACT: A whole-organ regeneration approach, using a decellularised xenogeneic liver as a scaffold for the construction of a transplantable liver was recently reported. Deriving suitable scaffolds was the first step towards clinical application; however, effective recellularisation remains to be achieved. This report presents a strategy for the improvement of the recellularisation process, using novel cell-seeding technique and cell source. We evaluated recellularised liver grafts repopulated through the portal vein or the biliary duct with mice adult hepatocytes or E14.5 foetal hepatocytes. More than 80% of the cells seeded through the biliary tree entered the parenchyma beyond the ductule-lining matrix barrier and distributed throughout the liver lobule. In contrast, about 20% of the cells seede

SUBMITTER: Ogiso S 

PROVIDER: S-EPMC5073336 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature