Hyaluronan coating improves liver engraftment of transplanted human biliary tree stem/progenitor cells.

ABSTRACT:

Background

Cell therapy of liver diseases with human biliary tree stem cells (hBTSCs) is biased by low engraftment efficiency. Coating the hBTSCs with hyaluronans (HAs), the primary constituents of all stem cell niches, could facilitate cell survival, proliferation, and, specifically, liver engraftment given that HAs are cleared selectively by the liver.

Methods

We developed a fast and easy method to coat hBTSCs with HA and assessed the effects of HA-coating on cell properties in vitro and in vivo.

Results

The HA coating markedly improved the viability, colony formation, and population doubling of hBTSCs in primary cultures, and resulted in a higher expression of integrins that mediate cell attachment to matrix components. When HA-coated hBTSCs were transplanted via t

SUBMITTER: Nevi L 

PROVIDER: S-EPMC5360089 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature