Endothelin-Mediated Changes in Gene Expression in Isolated Purified Rat Retinal Ganglion Cells.

ABSTRACT:

Purpose

A growing body of evidence suggests that the vasoactive peptides endothelins (ETs) and their receptors (primarily the ETB receptor) are contributors to neurodegeneration in glaucoma. However, actions of ETs in retinal ganglion cells (RGCs) are not fully understood. The purpose of this study was to determine the effects of ETs on gene expression in primary RGCs.

Methods

Primary RGCs isolated from rat pups were treated with 100 nM of ET-1, ET-2, or ET-3 for 24 hours. Total RNA was extracted followed by cDNA synthesis. Changes in gene expression in RGCs were detected using Affymetrix Rat Genome 230 2.0 microarray and categorized by DAVID analysis. Real-time PCR was used to validate gene expression, and immunocytochemistry and immunoblotting to confirm the protein expres

SUBMITTER: He S 

PROVIDER: S-EPMC5102497 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature