Refined purification strategy for reliable proteomic profiling of HDL_2/3: Impact on proteomic complexity.

ABSTRACT: Proteomics have extended the list of high-density lipoprotein (HDL) associated proteins to about 90. One of the major issues of global protein characterization is establishing specificity of association as opposed to contamination, a fact which has never been addressed for isolated HDL. We have developed a refined purification strategy to isolate HDL by density, followed by purification by size to generate "highly purified" fractions of HDL_2/3, which allow the reliable quantification of the HDL proteome for biomarker discovery. Mass spectrometry analysis revealed that the proteome of HDL_2/3 is composed of 10-16 different proteins, which is in striking contrast to previous reports. Importantly, proteomic analysis revealed that many proteins which have recently been des

SUBMITTER: Holzer M 

PROVIDER: S-EPMC5137140 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature