Low-dose fixed-target serial synchrotron crystallography.

ABSTRACT: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.

SUBMITTER: Owen RL 

PROVIDER: S-EPMC5379936 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature