Low-dose fixed-target serial synchrotron crystallography.
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ABSTRACT:
SUBMITTER: Owen RL
PROVIDER: S-EPMC5379936 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
Low-dose fixed-target serial synchrotron crystallography.
Owen Robin L RL Axford Danny D Sherrell Darren A DA Kuo Anling A Ernst Oliver P OP Schulz Eike C EC Miller R J Dwayne RJ Mueller-Werkmeister Henrike M HM
Acta crystallographica. Section D, Structural biology 20170331 Pt 4
The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput seri ...[more]
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