OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC BACILL US SUBTILIS.

ABSTRACT:

Background

Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production.

Materials and methods

Thermophilic amylase producing bacterium was isolated from local hot water-springs in Gazan city Saudi Arabia.

Results

Phylogenetic analysis of 16 S rRNA sequence for the strain revealed that the strain have the same sequence of Bacillus subtilis. Maximum amylase production was observed, when B. subtilis cultured in medium containing starch at concentration 0.5%, and 10 g/L peptones as nitro

SUBMITTER: Al-Johani NB 

PROVIDER: S-EPMC5411881 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature