Dataset Information

OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC BACILL US SUBTILIS.

ABSTRACT:

Background

Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production.

Materials and methods

Thermophilic amylase producing bacterium was isolated from local hot water-springs in Gazan city Saudi Arabia.

Results

Phylogenetic analysis of 16 S rRNA sequence for the strain revealed that the strain have the same sequence of Bacillus subtilis. Maximum amylase production was observed, when B. subtilis cultured in medium containing starch at concentration 0.5%, and 10 g/L peptones as nitrogen source at pH 8.5 in when it was incubated for 48 h at 45°C.

Conclusion

An amylase-producing bacterium were isolated from hot-spring water and was identified as B. subtilis. Amylase produced from B.subtilis had optimum temperature 45°C and pH 8.5 in shaking media.

SUBMITTER: Al-Johani NB

PROVIDER: S-EPMC5411881 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Publications

OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC <i>BACILL US SUBTILIS</i>.

Al-Johani Nuha Bakeet NB Al-Seeni Madeha N MN Ahmed Youssri Mohamed YM

African journal of traditional, complementary, and alternative medicines : AJTCAM 20161123 1

<h4>Background</h4>Starch-degrading amylase enzyme is important in biotechnological applications as food, fermentation, textile, paper and pharmaceutical purposes. The aim of current study to isolate alkaline thermostable α-amylase bacteria and then study the composition of medium and culture conditions to optimize cells growth and a-amylase production.<h4>Materials and methods</h4>Thermophilic amylase producing bacterium was isolated from local hot water-springs in Gazan city Saudi Arabia.<h4>R ...[more]

PMID: 28480407

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