Improved Xylose Metabolism by a CYC8 Mutant of Saccharomyces cerevisiae.

ABSTRACT: Engineering Saccharomyces cerevisiae for the utilization of pentose sugars is an important goal for the production of second-generation bioethanol and biochemicals. However, S. cerevisiae lacks specific pentose transporters, and in the presence of glucose, pentoses enter the cell inefficiently via endogenous hexose transporters (HXTs). By means of in vivo engineering, we have developed a quadruple hexokinase deletion mutant of S. cerevisiae that evolved into a strain that efficiently utilizes d-xylose in the presence of high d-glucose concentrations. A genome sequence analysis revealed a mutation (Y353C) in the general corepressor CYC8, or SSN6, which was found to be responsible for the phenotype when introduced individually in the nonevolved strai

SUBMITTER: Nijland JG 

PROVIDER: S-EPMC5440722 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature