Molecular Proteomic Characterization of a Pediatric Medulloblastoma Xenograft.

ABSTRACT:

Background/aim

Medulloblastoma (MBL), an archetypal primitive neuroectodermal tumor of the cerebellum, is the most common pediatric central nervous system malignancy representing approximately 20% of all childhood brain tumors. Herein, we report on a new xenotransplantable tumor cell line, derived from a 6-year-old female patient with cerebellar medulloblastoma, and the completele proteome molecular characterization of subsequent tumors from MBL xenotrasplanted mice.

Materials and methods

Tumors were grown in nude mice as subcutaneous xenografts (MBLX) composed of small round cells with hyperchromatic nuclei and scant cytoplasm. Tumor specimen were extracted from animals upon their sacrifice and their molecular proteomic content was analyzed by 2-DE coupled to MALDI-TOF MS a

SUBMITTER: Tsangaris GT 

PROVIDER: S-EPMC5572304 | biostudies-literature | 2017 Jul-Aug

REPOSITORIES: biostudies-literature