A novel intracellular pool of LFA-1 is critical for asymmetric CD8⁺ T cell activation and differentiation.

ABSTRACT: The integrin lymphocyte function-associated antigen 1 (LFA-1; CD11a/CD18) is a key T cell adhesion receptor that mediates stable interactions with antigen-presenting cell (APC), as well as chemokine-mediated migration. Using our newly generated CD11a-mYFP knock-in mice, we discovered that naive CD8⁺ T cells reserve a significant intracellular pool of LFA-1 in the uropod during migration. Intracellular LFA-1 quickly translocated to the cell surface with antigenic stimulus. Importantly, the redistribution of intracellular LFA-1 at the contact with APC was maintained during cell division and led to an unequal inheritance of LFA-1 in divided T cells. The daughter CD8⁺ T cells with disparate LFA-1 expression showed different patterns of migration on ICAM-1, APC interaction

SUBMITTER: Capece T 

PROVIDER: S-EPMC5674876 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature