DNA sequence-dependent epigenetic inheritance of gene silencing and histone H3K9 methylation.

ABSTRACT: Epigenetic inheritance mechanisms play fundamental roles in maintaining cellular memory of gene expression states. In fission yeast, histone H3 lysine 9 (H3K9) is methylated (H3K9me) at heterochromatic domains. These domains can be epigenetically inherited when epe1⁺ , encoding an enzyme that promotes H3K9 demethylation, is deleted. How native epigenetic states are stably maintained in epe1⁺ cells remains unknown. Here, we developed a system to examine the role of DNA sequence and genomic context in propagation of a cis-heritable H3K9me-dependent silenced state. We show that in epe1⁺ cells, in addition to sequence-independent mechanisms that propagate H3K9me, epigenetic inheritance of silencing requires binding sites for sequence-depend

SUBMITTER: Wang X 

PROVIDER: S-EPMC5718040 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature