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ABSTRACT: Introduction
The role of MerTK has not been assessed in gastric cancer (GC). The aim of this study was to identify a subgroup of GC patients with MerTK tumor overexpression, and to evaluate MerTK as a potential therapeutic target in this disease.Methods
Protein and mRNA expression of MerTK were evaluated, and other various in vitro analyses including shRNA transfection, cell cycle anslysis, MTS assay and colony forming assay were carried out with GC cell lines and GC patient-derived cells (PDCs).Results
shRNA-mediated knockdown of MerTK resulted in inhibition of cell growth, as well as increased cellular apoptosis in MerTK positive GC cells. Out of 192 GC patients, 16 (8.3%) patients showed strong protein expression and they had a significantly shorter overall survival compared to those with no MerTK expression. In 54 cases of GC PDCs, 4 cases (7.4%) showed mRNA overexpression, which was comparable to the protein expression rate. When we administered UNC1062, a novel MerTK-selective small molecular tyrosine kinase inhibitor, proliferation of MerTK overexpressing GC cells and PDCs were considerably inhibited.Conclusion
MerTK may be involved in GC carcinogenesis, and it could be a potential novel therapeutic target in GC patients.
SUBMITTER: Yi JH
PROVIDER: S-EPMC5722512 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Yi Jun Ho JH Jang Jiryeon J Cho Jeonghee J Do In-Gu IG Hong Mineui M Kim Seung Tae ST Kim Kyoung-Mee KM Lee Sujin S Park Se Hoon SH Park Joon Oh JO Park Young Suk YS Kang Won Ki WK Lim Ho Yeong HY Lee Jeeyun J
Oncotarget 20150420 57
<h4>Introduction</h4>The role of MerTK has not been assessed in gastric cancer (GC). The aim of this study was to identify a subgroup of GC patients with MerTK tumor overexpression, and to evaluate MerTK as a potential therapeutic target in this disease.<h4>Methods</h4>Protein and mRNA expression of MerTK were evaluated, and other various <i>in vitro</i> analyses including shRNA transfection, cell cycle anslysis, MTS assay and colony forming assay were carried out with GC cell lines and GC patie ...[more]