The influence of glyoxalase 1 gene polymorphism on its expression at different stages of breast cancer in Egyptian women.

ABSTRACT:

Aim

To assess the association of GLO1 C332C gene polymorphism with breast cancer risk at different stages of the disease and to investigate the effect of this gene polymorphism on its mRNA expression and enzyme activity.

Methods

GLO1 C332C gene polymorphism was analyzed by PCR-RFLP in 100 healthy controls and 200 patients with breast cancer (100 patients with stage I & II and 100 patients with stage III & IV). GLO1 mRNA expression was measured by real time PCR. Serum GLO1 enzyme activity was measured colorimetrically.

Results

GLO1 A allele was associated with increased risk of breast cancer [OR (95%CI)= 2.8(1.9-4.1), P < 0.001]. Its frequency was significantly higher among advanced stages of breast cancer compared with localized tumors (OR (95%CI)= 1.9(1.3-2.9)

SUBMITTER: Abdul-Maksoud RS 

PROVIDER: S-EPMC5755725 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature