Widespread Translational Remodeling during Human Neuronal Differentiation.

ABSTRACT: Faithful cellular differentiation requires temporally precise activation of gene expression programs, which are coordinated at the transcriptional and translational levels. Neurons express the most complex set of mRNAs of any human tissue, but translational changes during neuronal differentiation remain incompletely understood. Here, we induced forebrain neuronal differentiation of human embryonic stem cells (hESCs) and measured genome-wide RNA and translation levels with transcript-isoform resolution. We found that thousands of genes change translation status during differentiation without a corresponding change in RNA level. Specifically, we identified mTOR signaling as a key driver for elevated translation of translation-related genes in hESCs. In contrast, translational repression in a

SUBMITTER: Blair JD 

PROVIDER: S-EPMC5759054 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature