A Remote Assay for Measuring Canine Platelet Activation and the Inhibitory Effects of Antiplatelet Agents.

ABSTRACT:

Background

Antiplatelet medications are increasingly used in dogs. Remote analysis of platelet activity is challenging, limiting assessment of antiplatelet drug efficacy.

Hypothesis/objectives

To evaluate a method used in humans for stimulation and remote analysis of canine platelet activity.

Animals

Forty-five dogs of various ages without a coagulopathy or thrombocytopenia. Six were receiving antiplatelet medication.

Methods

Prospective observational study. Platelets were stimulated with combinations of arachidonic acid (AA) and epinephrine (Epi) or adenosine diphosphate (ADP) and the thromboxane A₂ -mimetic U46619 (U4). PAMFix was added to the blood samples to facilitate delayed analysis of platelet activity. Activity was assessed by flow cytometri

SUBMITTER: Dunning M 

PROVIDER: S-EPMC5787215 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature