Plasma QconCATs reveal a gender-specific proteomic signature in apheresis platelet plasma supernatants.

ABSTRACT: Clinical translation of proteomic technologies is often hampered by technical limitations, including inter-laboratory inconsistencies of label-free derived relative quantification, time-consuming analytical approaches and the subsequent challenge of performing proteomic analyses on large cohorts of subjects. Here we introduce plasma QconCAT-based targeted proteomics, an approach that allows the simultaneous absolute quantitation down to the picogram level of hundreds of proteins in a single liquid chromatography-selected reaction monitoring mass spectrometry run. We demonstrate the robustness of the approach by analyzing apheresis platelet concentrate supernatants at storage day 1 and the end of the shelf life for this blood-derived therapeutic, day 5. The targeted approach was repeatable

SUBMITTER: Dzieciatkowska M 

PROVIDER: S-EPMC5794015 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature