Ontology highlight
ABSTRACT:
SUBMITTER: Tilak MK
PROVIDER: S-EPMC5808798 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Tilak Marie-Ka MK Botero-Castro Fidel F Galtier Nicolas N Nabholz Benoit B
Genome biology and evolution 20180201 2
Standard Illumina libraries are biased toward sequences of intermediate GC-content. This results in an underrepresentation of GC-rich regions in sequencing projects of genomes with heterogeneous base composition, such as mammals and birds. We developed a simple, cost-effective protocol to enrich sheared genomic DNA in its GC-rich fraction by subtracting AT-rich DNA. This was achieved by heating DNA up to 90 °C before applying Illumina library preparation. We tested the new approach on chicken DN ...[more]