Dynamic light sheet generation and fluorescence imaging behind turbid media.

ABSTRACT:

Background

Light sheet microscopy became a popular tool allowing fast imaging with reduced out of focus light. However, when light penetrates turbid media such as biological tissues, multiple scattering scrambles the illumination into a speckle pattern and severely challenges conventional fluorescence imaging with focused light or with a light sheet. In this article, we present generation of light sheet type illumination patterns despite scattering.

Methods

We optimize the wave-front of the incoming light to transform the speckle pattern behind the scattering layer into a light sheet within the region of interest. We utilize a fast spatial light modulator for phase modulation and a genetic optimization algorithm. The light pattern behind the scattering layer is detected via

SUBMITTER: Schneider J 

PROVIDER: S-EPMC5846999 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature