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ABSTRACT: Aim
This study explored the effects of blood flow restriction (BFR) on mRNA responses of PGC-1? (total, 1?1, and 1?4) and Na+ ,K+ -ATPase isoforms (NKA; ?1-3 , ?1-3 , and FXYD1) to an interval running session and determined whether these effects were related to increased oxidative stress, hypoxia, and fibre type-specific AMPK and CaMKII signalling, in human skeletal muscle.Methods
In a randomized, crossover fashion, 8 healthy men (26 ± 5 year and 57.4 ± 6.3 mL kg-1 min-1 ) completed 3 exercise sessions: without (CON) or with blood flow restriction (BFR), or in systemic hypoxia (HYP, ~3250 m). A muscle sample was collected before (Pre) and after exercise (+0 hour, +3 hours) to quantify mRNA, indicators of oxidative stress (HSP27 protein in type I and II fibres, and catalase and HSP70 mRNA), metabolites, and ?-AMPK Thr172 /?-AMPK, ACC Ser221 /ACC, CaMKII Thr287 /CaMKII, and PLBSer16 /PLB ratios in type I and II fibres.Results
Muscle hypoxia (assessed by near-infrared spectroscopy) was matched between BFR and HYP, which was higher than CON (~90% vs ~70%; P < .05). The mRNA levels of FXYD1 and PGC-1? isoforms (1?1 and 1?4) increased in BFR only (P < .05) and were associated with increases in indicators of oxidative stress and type I fibre ACC Ser221 /ACC ratio, but dissociated from muscle hypoxia, lactate, and CaMKII signalling.Conclusion
Blood flow restriction augmented exercise-induced increases in muscle FXYD1 and PGC-1? mRNA in men. This effect was related to increased oxidative stress and fibre type-dependent AMPK signalling, but unrelated to the severity of muscle hypoxia, lactate accumulation, and modulation of fibre type-specific CaMKII signalling.
SUBMITTER: Christiansen D
PROVIDER: S-EPMC5969286 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Christiansen D D Murphy R M RM Bangsbo J J Stathis C G CG Bishop D J DJ
Acta physiologica (Oxford, England) 20180227 2
<h4>Aim</h4>This study explored the effects of blood flow restriction (BFR) on mRNA responses of PGC-1α (total, 1α1, and 1α4) and Na<sup>+</sup> ,K<sup>+</sup> -ATPase isoforms (NKA; α<sub>1-3</sub> , β<sub>1-3</sub> , and FXYD1) to an interval running session and determined whether these effects were related to increased oxidative stress, hypoxia, and fibre type-specific AMPK and CaMKII signalling, in human skeletal muscle.<h4>Methods</h4>In a randomized, crossover fashion, 8 healthy men (26 ± ...[more]