Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips.

ABSTRACT:

Background

Preservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies.

Results

The present study reported a novel biotechnology allowing cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips. Following cryopreservation by droplet-vitrification or encapsulation-dehydration, about 62-67% of shoot regrowth and 100% of ASGV cryopreservation were obtained. Although shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration, continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus conc

SUBMITTER: Wang MR 

PROVIDER: S-EPMC5996562 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature