Lipidomic profiling of extracellular vesicles derived from prostate and prostate cancer cell lines.

ABSTRACT:

Background

Extracellular vesicles (EVs) are produced and secreted from most cells of the body and can be recovered in biological fluids. Although there has been extensive characterisation of the protein and nucleic acid component of EVs, their lipidome has received little attention and may represent a unique and untapped source of biomarkers for prostate cancer diagnosis and prognosis.

Methods

EVs were isolated from non-tumourigenic (RWPE1), tumourigenic (NB26) and metastatic (PC-3) prostate cell lines. Lipids were extracted and subsequently used for targeted lipidomics analysis for the quantitation of molecular lipid species.

Results

A total of 187 molecular lipid species were quantitatively identified in EV samples showing differential abundance between RWPE1, NB26

SUBMITTER: Brzozowski JS 

PROVIDER: S-EPMC6128989 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature