Ontology highlight
ABSTRACT:
SUBMITTER: Bartesaghi A
PROVIDER: S-EPMC6129192 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20180510 6
The advent of direct electron detectors has enabled the routine use of single-particle cryo-electron microscopy (EM) approaches to determine structures of a variety of protein complexes at near-atomic resolution. Here, we report the development of methods to account for local variations in defocus and beam-induced drift, and the implementation of a data-driven dose compensation scheme that significantly improves the extraction of high-resolution information recorded during exposure of the specim ...[more]