Dataset Information

Comparative analysis of bla _KPC-2- and rmtB-carrying IncFII-family pKPC-LK30/pHN7A8 hybrid plasmids from Klebsiella pneumoniae CG258 strains disseminated among multiple Chinese hospitals.

ABSTRACT:

Background

We recently reported the complete sequence of a blaKPC-2- and rmtB-carrying IncFII-family plasmid p675920-1 with the pKPC-LK30/pHN7A8 hybrid structure. Comparative genomics of additional sequenced plasmids with similar hybrid structures and their prevalence in bla _KPC-carrying Klebsiella pneumoniae strains from China were investigated in this follow-up study.

Methods

A total of 51 bla _KPC-carrying K. pneumoniae strains were isolated from 2012 to 2016 from five Chinese hospitals and genotyped by multilocus sequence typing. The bla _KPC-carrying plasmids from four representative strains were sequenced and compared with p675920-1 and pCT-KPC. Plasmid transfer, carbapenemase activity determination, and bacterial antimicrobial susceptibility test were performed to characterize resistance phenotypes mediated by these plasmids. The prevalence of pCT-KPC-like plasmids in these bla _KPC-carrying K. pneumoniae strains was screened by PCR.

Result

The six KPC-encoding plasmids p1068-KPC, p20049-KPC, p12139-KPC and p64917-KPC (sequenced in this study) and p675920-1 and pCT-KPC slightly differed from one another due to deletion and acquisition of various backbone and accessory regions. Two major accessory resistance regions, which included the blaKPC-2 region harboring blaKPC-2 (carbapenem resistance) and blaSHV-12 (β-lactam resistance), and the MDR region carrying rmtB (aminoglycoside resistance), fosA3 (fosfomycin resistance), bla _TEM-1B (β-lactam resistance) and bla _CTX-M-65 (β-lactam resistance), were found in each of these six plasmids and exhibited several parallel evolution routes. The pCT-KPC-like plasmids were present in all the 51 K. pneumoniae isolates, all of which belonged to CG258.

Conclusion

There was clonal dissemination of K. pneumoniae CG258 strains, harboring bla _KPC-2- and rmtB-carrying IncFII-family pKPC-LK30/pHN7A8 hybrid plasmids, among multiple Chinese hospitals.

SUBMITTER: Shi L

PROVIDER: S-EPMC6188201 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Comparative analysis of bla KPC-2- and rmtB-carrying IncFII-family pKPC-LK30/pHN7A8 hybrid plasmids from Klebsiella pneumoniae CG258 strains disseminated among multiple Chinese hospitals.

Shi Lining L Feng Jiao J Zhan Zhe Z Zhao Yuzong Y Zhou Haijian H Mao Haifeng H Gao Yingjie Y Zhang Ying Y Yin Zhe Z Gao Bo B Tong Yigang Y Luo Yanping Y Zhang Defu D Zhou Dongsheng D

Infection and drug resistance 20181011

<h4>Background</h4>We recently reported the complete sequence of a blaKPC-2- and rmtB-carrying IncFII-family plasmid p675920-1 with the pKPC-LK30/pHN7A8 hybrid structure. Comparative genomics of additional sequenced plasmids with similar hybrid structures and their prevalence in bla KPC-carrying Klebsiella pneumoniae strains from China were investigated in this follow-up study.<h4>Methods</h4>A total of 51 bla KPC-carrying K. pneumoniae ...[more]

PMID: 30349335

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